CHEK1 CLIA Kit for Human(Homo sapiens) | EIAab.comCHEK1 CLIA Kit for Human(Homo sapiens)

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CHEK1 (Gene name),

Serine/threonine-protein kinase Chk1 (Protein name ), CHK1_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

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Gene name:

CHEK1(CHK1);

Protein name:

Serine/threonine-protein kinase Chk1;

Alternative:

Cell cycle checkpoint kinase;CHK1 checkpoint homolog;Checkpoint kinase-1;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Interacts with BRCA1, CLSPN, FBXO6, PPM1D, RAD51, TIMELESS, XPO1/CRM1 and YWHAZ/14-3-3 zeta. Interacts with FEM1B.

Function:

Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation. Phosphorylates NEK6 and NEK11. Phosphorylation of CDC25A at Ser-76 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11.

Subcellular Location:

Nucleus Cytoplasm Cytoplasm cytoskeleton centrosome Nuclear export is mediated at least in part by XPO1/CRM1. Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B.

Protein Attributes:

Sequence length:

476

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

476:

FKRHFLKIKG | KLIDIVSSQK | IWLPAT

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

STRING:

9606.ENSP00000278916

SMR:

O14757

MIM:

603078

Pfam:

PF00069

KEGG:

hsa:1111

UniGene:

Hs.24529

String:

O14757

Uniprot:

O14757

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ELISA Kit for Human Serine/threonine-protein kinase Chk1

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E11379m

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96T

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ELISA Kit for Human Serine/threonine-protein kinase Chk1

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E11379c

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96T

ELISA Kit for Human Serine/threonine-protein kinase Chk1

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E11379r

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ELISA Kit for Human Serine/threonine-protein kinase Chk1

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E11379h

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CLIA Kit for Human Serine/threonine-protein kinase Chk1

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U11379r

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96T

CLIA Kit for Human Serine/threonine-protein kinase Chk1

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U11379h

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CLIA Kit for Human Serine/threonine-protein kinase Chk1

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U11379c

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CLIA Kit for Human Serine/threonine-protein kinase Chk1

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U11379m

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Polyclonal Antibody for Human Serine/threonine-protein kinase Chk1

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P11379Rb-h

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40ug/0.2ml

Polyclonal Antibody for Human Serine/threonine-protein kinase Chk1

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P11379Rb-r

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Polyclonal Antibody for Human Serine/threonine-protein kinase Chk1

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P11379Rb-m

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Polyclonal Antibody for Human Serine/threonine-protein kinase Chk1

Monoclonal Antibody for Human Serine/threonine-protein kinase Chk1

Protein for Human Serine/threonine-protein kinase Chk1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25."
Sanchez Y. , Wong C. , Thoma R.S. , Richman R. , Wu Z. , Piwnica-Worms H. , Elledge S.J.
Science277:1497-1501(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);FUNCTION IN PHOSPHORYLATION OF CDC25A; CDC25B AND CDC25C;INTERACTION WITH CDC25A; CDC25B AND CDC25C;SUBCELLULAR LOCATION;TISSUE SPECIFICITY;MUTAGENESIS OF ASP-130;VARIANT VAL-471
2.

"Atm-dependent interactions of a mammalian chk1 homolog with meiotic chromosomes."
Flaggs G. , Plug A.W. , Dunks K.M. , Mundt K.E. , Ford J.C. , Quiggle M.R.E. , Taylor E.M. , Westphal C.H. , Ashley T. , Hoekstra M.F. , Carr A.M.
Curr. Biol.7:977-986(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);TISSUE SPECIFICITY;SUBCELLULAR LOCATION;VARIANT VAL-471
3.

"Analysis of the candidate target genes for mutation in microsatellite instability-positive cancers of the colorectum, stomach, and endometrium."
Semba S. , Ouyang H. , Han S.-Y. , Kato Y. , Horii A.
Int. J. Oncol.16:731-737(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT VAL-471
4.

"Checkpoint kinase 1 (Chk1)-short is a splice variant and endogenous inhibitor of Chk1 that regulates cell cycle and DNA damage checkpoints."
Pabla N. , Bhatt K. , Dong Z.
Proc. Natl. Acad. Sci. U.S.A.109:197-202(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2);ALTERNATIVE SPLICING
tissue: Fetal thymus.
5.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3);VARIANT VAL-471
tissue: Brain.
tissue: Testis.
6.

NIEHS SNPs program
Submitted (2002-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS GLN-156 AND VAL-471
7.

"Human protein factory for converting the transcriptome into an in vitro-expressed proteome."
Goshima N. , Kawamura Y. , Fukumoto A. , Miura A. , Honma R. , Satoh R. , Wakamatsu A. , Yamamoto J. , Kimura K. , Nishikawa T. , Andoh T. , Iida Y. , Ishikawa K. , Ito E. , Kagawa N. , Kaminaga C. , Kanehori K. , Kawakami B. , more...
Nat. Methods5:1011-1017(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1);VARIANT VAL-471
8.

"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D. , Noguchi H. , Totoki Y. , Toyoda A. , Kuroki Y. , Dewar K. , Lloyd C. , Itoh T. , Takeda T. , Kim D.-W. , She X. , Barlow K.F. , Bloom T. , Bruford E. , Chang J.L. , Cuomo C.A. , Eichler E. , FitzGerald M.G. , more...
Nature440:497-500(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
9.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA];VARIANT VAL-471
10.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1);VARIANT VAL-471
tissue: Bone marrow.
tissue: Muscle.
11.

"The human homologs of checkpoint kinases Chk1 and Cds1 (Chk2) phosphorylate p53 at multiple DNA damage-inducible sites."
Shieh S.-Y. , Ahn J. , Tamai K. , Taya Y. , Prives C.
Genes Dev.14:289-300(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF TP53;MUTAGENESIS OF ASP-130
12.

Shieh S.-Y. , Ahn J. , Tamai K. , Taya Y. , Prives C.
Genes Dev.14:750-750(2000)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM
13.

"Chk1 is an essential kinase that is regulated by Atr and required for the G(2)/M DNA damage checkpoint."
Liu Q. , Guntuku S. , Cui X.-S. , Matsuoka S. , Cortez D. , Tamai K. , Luo G. , Carattini-Rivera S. , DeMayo F. , Bradley A. , Donehower L.A. , Elledge S.J.
Genes Dev.14:1448-1459(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-345 BY ATR
14.

"Activation of mammalian Chk1 during DNA replication arrest: a role for Chk1 in the intra-S phase checkpoint monitoring replication origin firing."
Feijoo C. , Hall-Jackson C. , Wu R. , Jenkins D. , Leitch J. , Gilbert D.M. , Smythe C.
J. Cell Biol.154:913-923(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN DNA REPLICATION;PHOSPHORYLATION BY ATR;MUTAGENESIS OF ASP-130
15.

"ATR-mediated checkpoint pathways regulate phosphorylation and activation of human Chk1."
Zhao H. , Piwnica-Worms H.
Mol. Cell. Biol.21:4129-4139(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-317 AND SER-345;MUTAGENESIS OF ASP-130; SER-317; SER-345; SER-357; SER-366 AND SER-468
16.

"Determination of substrate motifs for human Chk1 and hCds1/Chk2 by the oriented peptide library approach."
O'Neill T. , Giarratani L. , Chen P. , Iyer L. , Lee C.-H. , Bobiak M. , Kanai F. , Zhou B.-B. , Chung J.H. , Rathbun G.A.
J. Biol. Chem.277:16102-16115(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBSTRATE SPECIFICITY;MUTAGENESIS OF ASP-130
17.

O'Neill T. , Giarratani L. , Chen P. , Iyer L. , Lee C.-H. , Bobiak M. , Kanai F. , Zhou B.-B. , Chung J.H. , Rathbun G.A.
J. Biol. Chem.277:35776-35777(2002)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM
18.

"An ATR- and Chk1-dependent S checkpoint inhibits replicon initiation following UVC-induced DNA damage."
Heffernan T.P. , Simpson D.A. , Frank A.R. , Heinloth A.N. , Paules R.S. , Cordeiro-Stone M. , Kaufmann W.K.
Mol. Cell. Biol.22:8552-8561(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN DNA DAMAGE RESPONSE;PHOSPHORYLATION AT SER-317 AND SER-345;MUTAGENESIS OF LYS-38
19.

"BRCA1 regulates the G2/M checkpoint by activating Chk1 kinase upon DNA damage."
Yarden R.I. , Pardo-Reoyo S. , Sgagias M. , Cowan K.H. , Brody L.C.
Nat. Genet.30:285-289(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;INTERACTION WITH BRCA1
20.

"Disruption of the checkpoint kinase 1/cell division cycle 25A pathway abrogates ionizing radiation-induced S and G2 checkpoints."
Zhao H. , Watkins J.L. , Piwnica-Worms H.
Proc. Natl. Acad. Sci. U.S.A.99:14795-14800(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN DNA DAMAGE RESPONSE
21.

"Chk1 regulates the S phase checkpoint by coupling the physiological turnover and ionizing radiation-induced accelerated proteolysis of Cdc25A."
Soerensen C.S. , Syljuaesen R.G. , Falck J. , Schroeder T. , Roennstrand L. , Khanna K.K. , Zhou B.-B. , Bartek J. , Lukas J.
Cancer Cell3:247-258(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN CDC25A TURNOVER;PHOSPHORYLATION AT SER-317 AND SER-345;MUTAGENESIS OF SER-317 AND SER-345
22.

"Human tousled like kinases are targeted by an ATM- and Chk1-dependent DNA damage checkpoint."
Groth A. , Lukas J. , Nigg E.A. , Sillje H.H.W. , Wernstedt C. , Bartek J. , Hansen K.
EMBO J.22:1676-1687(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF TLK1;PHOSPHORYLATION AT SER-317
23.

"SCFbeta-TRCP links Chk1 signaling to degradation of the Cdc25A protein phosphatase."
Jin J. , Shirogane T. , Xu L. , Nalepa G. , Qin J. , Elledge S.J. , Harper J.W.
Genes Dev.17:3062-3074(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN CDC25A TURNOVER;MUTAGENESIS OF ASP-130
24.

"Ataxia-telangiectasia-mutated (ATM) and NBS1-dependent phosphorylation of Chk1 on ser-317 in response to ionizing radiation."
Gatei M. , Sloper K. , Soerensen C. , Syljuaesen R. , Falck J. , Hobson K. , Savage K. , Lukas J. , Zhou B.-B. , Bartek J. , Khanna K.K.
J. Biol. Chem.278:14806-14811(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-317;MUTAGENESIS OF ASP-130; SER-317 AND SER-345
25.

"Chk1 mediates S and G2 arrests through Cdc25A degradation in response to DNA-damaging agents."
Xiao Z. , Chen Z. , Gunasekera A.H. , Sowin T.J. , Rosenberg S.H. , Fesik S. , Zhang H.
J. Biol. Chem.278:21767-21773(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN CDC25A TURNOVER;PHOSPHORYLATION AT SER-345
26.

"Regulation of Chk1 includes chromatin association and 14-3-3 binding following phosphorylation on ser-345."
Jiang K. , Pereira E. , Maxfield M. , Russell B. , Goudelock D.M. , Sanchez Y.
J. Biol. Chem.278:25207-25217(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH YWHAZ AND XPO1;SUBCELLULAR LOCATION;ASSOCIATION WITH CHROMATIN;PHOSPHORYLATION AT SER-317 AND SER-345;MUTAGENESIS OF SER-317; PHE-344; SER-345 AND MET-353
27.

"Phosphorylation at serine 75 is required for UV-mediated degradation of human Cdc25A phosphatase at the S-phase checkpoint."
Hassepass I. , Voit R. , Hoffmann I.
J. Biol. Chem.278:29824-29829(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN CDC25A TURNOVER
28.

"Human claspin is required for replication checkpoint control."
Chini C.C.S. , Chen J.
J. Biol. Chem.278:30057-30062(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CLSPN
29.

"Chk1 kinase negatively regulates mitotic function of Cdc25A phosphatase through 14-3-3 binding."
Chen M.-S. , Ryan C.E. , Piwnica-Worms H.
Mol. Cell. Biol.23:7488-7497(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN MITOSIS;FUNCTION IN PHOSPHORYLATION OF CDC25A
30.

"Suppression of tousled-like kinase activity after DNA damage or replication block requires ATM, NBS1 and Chk1."
Krause D.R. , Jonnalagadda J.C. , Gatei M.H. , Sillje H.H.W. , Zhou B.-B. , Nigg E.A. , Khanna K.
Oncogene22:5927-5937(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REGULATION OF TLK1
31.

"MSH2 and ATR form a signaling module and regulate two branches of the damage response to DNA methylation."
Wang Y. , Qin J.
Proc. Natl. Acad. Sci. U.S.A.100:15387-15392(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-317
32.

"The DNA crosslink-induced S-phase checkpoint depends on ATR-CHK1 and ATR-NBS1-FANCD2 pathways."
Pichierri P. , Rosselli F.
EMBO J.23:1178-1187(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;PHOSPHORYLATION AT SER-345
33.

"Differential mode of regulation of the checkpoint kinases CHK1 and CHK2 by their regulatory domains."
Ng C.-P. , Lee H.C. , Ho C.W. , Arooz T. , Siu W.Y. , Lau A. , Poon R.Y.C.
J. Biol. Chem.279:8808-8819(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DOMAIN;MITOTIC PHOSPHORYLATION;PHOSPHORYLATION AT SER-345;MUTAGENESIS OF LYS-38
34.

"Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase."
Kraemer A. , Mailand N. , Lukas C. , Syljuaesen R.G. , Wilkinson C.J. , Nigg E.A. , Bartek J. , Lukas J.
Nat. Cell Biol.6:884-891(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN MITOSIS;SUBCELLULAR LOCATION;MUTAGENESIS OF ASP-130
35.

"DNA-dependent phosphorylation of Chk1 and claspin in a human cell-free system."
Clarke C.A.L. , Clarke P.R.
Biochem. J.388:705-712(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CLSPN;PHOSPHORYLATION AT SER-296; SER-317 AND SER-345
36.

"Lack of PTEN sequesters CHK1 and initiates genetic instability."
Puc J. , Keniry M. , Li H.S. , Pandita T.K. , Choudhury A.D. , Memeo L. , Mansukhani M. , Murty V.V.V.S. , Gaciong Z. , Meek S.E.M. , Piwnica-Worms H. , Hibshoosh H. , Parsons R.
Cancer Cell7:193-204(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION
37.

"PPM1D dephosphorylates Chk1 and p53 and abrogates cell cycle checkpoints."
Lu X. , Nannenga B. , Donehower L.A.
Genes Dev.19:1162-1174(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PPM1D;PHOSPHORYLATION AT SER-317 AND SER-345;DEPHOSPHORYLATION BY PPM1D
38.

"p53 C-terminal phosphorylation by CHK1 and CHK2 participates in the regulation of DNA-damage-induced C-terminal acetylation."
Ou Y.-H. , Chung P.-H. , Sun T.-P. , Shieh S.-Y.
Mol. Biol. Cell16:1684-1695(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF TP53;FUNCTION IN TP53-DEPENDENT TRANSCRIPTION
39.

"Coupling of human circadian and cell cycles by the timeless protein."
Uensal-Kacmaz K. , Mullen T.E. , Kaufmann W.K. , Sancar A.
Mol. Cell. Biol.25:3109-3116(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TIMELESS
40.

"The cell-cycle checkpoint kinase Chk1 is required for mammalian homologous recombination repair."
Soerensen C.S. , Hansen L.T. , Dziegielewski J. , Syljuaesen R.G. , Lundin C. , Bartek J. , Helleday T.
Nat. Cell Biol.7:195-201(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN HOMOLOGOUS RECOMBINATION REPAIR;FUNCTION IN PHOSPHORYLATION OF RAD51;INTERACTION WITH RAD51;MUTAGENESIS OF SER-317 AND SER-345
41.

"DNA damage-induced mitotic catastrophe is mediated by the Chk1-dependent mitotic exit DNA damage checkpoint."
Huang X. , Tran T. , Zhang L. , Hatcher R. , Zhang P.
Proc. Natl. Acad. Sci. U.S.A.102:1065-1070(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN MITOTIC EXIT;PHOSPHORYLATION AT SER-345
42.

"14-3-3gamma binds to MDMX that is phosphorylated by UV-activated Chk1, resulting in p53 activation."
Jin Y. , Dai M.S. , Lu S.Z. , Xu Y. , Luo Z. , Zhao Y. , Lu H.
EMBO J.25:1207-1218(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN TP53 ACTIVATION;FUNCTION IN PHOSPHORYLATION OF MDM4
43.

"Repeated phosphopeptide motifs in human Claspin are phosphorylated by Chk1 and mediate Claspin function."
Chini C.C. , Chen J.
J. Biol. Chem.281:33276-33282(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF CLSPN;INTERACTION WITH CLSPN
44.

"Phosphorylation of pRB at Ser612 by Chk1/2 leads to a complex between pRB and E2F-1 after DNA damage."
Inoue Y. , Kitagawa M. , Taya Y.
EMBO J.26:2083-2093(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF RB1
45.

"Chk1-mediated phosphorylation of FANCE is required for the Fanconi anemia/BRCA pathway."
Wang X. , Kennedy R.D. , Ray K. , Stuckert P. , Ellenberger T. , D'Andrea A.D.
Mol. Cell. Biol.27:3098-3108(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN DNA CROSS-LINKS REPAIR;FUNCTION IN PHOSPHORYLATION OF FANCE
46.

"Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry."
Denis N.J. , Vasilescu J. , Lambert J.-P. , Smith J.C. , Figeys D.
Proteomics7:868-874(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-436
tissue: Mammary cancer.
47.

"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S. , Ballif B.A. , Smogorzewska A. , McDonald E.R. III , Hurov K.E. , Luo J. , Bakalarski C.E. , Zhao Z. , Solimini N. , Lerenthal Y. , Shiloh Y. , Gygi S.P. , Elledge S.J.
Science316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Embryonic kidney.
48.

"Chk1 suppresses a caspase-2 apoptotic response to DNA damage that bypasses p53, Bcl-2, and caspase-3."
Sidi S. , Sanda T. , Kennedy R.D. , Hagen A.T. , Jette C.A. , Hoffmans R. , Pascual J. , Imamura S. , Kishi S. , Amatruda J.F. , Kanki J.P. , Green D.R. , D'Andrea A.A. , Look A.T.
Cell133:864-877(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN APOPTOSIS
49.

"Nek6 is involved in G2/M phase cell cycle arrest through DNA damage-induced phosphorylation."
Lee M.Y. , Kim H.J. , Kim M.A. , Jee H.J. , Kim A.J. , Bae Y.S. , Park J.I. , Chung J.H. , Yun J.
Cell Cycle7:2705-2709(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF NEK6
50.

"Chk1 and Claspin potentiate PCNA ubiquitination."
Yang X.H. , Shiotani B. , Classon M. , Zou L.
Genes Dev.22:1147-1152(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN REPLICATION FORK MAINTENANCE;FUNCTION IN PCNA UBIQUITINATION
51.

"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H. , Olsen J.V. , Bairlein M. , Gnad F. , Oppermann F.S. , Korner R. , Greff Z. , Keri G. , Stemmann O. , Mann M.
Mol. Cell31:438-448(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
52.

"The checkpoint kinases Chk1 and Chk2 regulate the functional associations between hBRCA2 and Rad51 in response to DNA damage."
Bahassi E.M. , Ovesen J.L. , Riesenberg A.L. , Bernstein W.Z. , Hasty P.E. , Stambrook P.J.
Oncogene27:3977-3985(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN RAD51-MEDIATED DNA REPAIR;FUNCTION IN PHOSPHORYLATION OF BRCA2 AND RAD51
53.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-286; SER-296 AND SER-301;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
54.

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
55.

"The F box protein Fbx6 regulates Chk1 stability and cellular sensitivity to replication stress."
Zhang Y.-W. , Brognard J. , Coughlin C. , You Z. , Dolled-Filhart M. , Aslanian A. , Manning G. , Abraham R.T. , Hunter T.
Mol. Cell35:442-453(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-345;UBIQUITINATION AT LYS-436;INTERACTION WITH FBXO6;MUTAGENESIS OF SER-345; ARG-372; ARG-376; ARG-379 AND LYS-436
56.

"Large-scale proteomics analysis of the human kinome."
Oppermann F.S. , Gnad F. , Olsen J.V. , Hornberger R. , Greff Z. , Keri G. , Mann M. , Daub H.
Mol. Cell. Proteomics8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-296;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
57.

"NEK11 regulates CDC25A degradation and the IR-induced G2/M checkpoint."
Melixetian M. , Klein D.K. , Soerensen C.S. , Helin K.
Nat. Cell Biol.11:1247-1253(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN NEK11 PHOSPHORYLATION
58.

"Human FEM1B is required for Rad9 recruitment and CHK1 activation in response to replication stress."
Sun T.P. , Shieh S.Y.
Oncogene28:1971-1981(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH FEM1B
59.

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V. , Lundgren D.H. , Hwang S.-I. , Rezaul K. , Wu L. , Eng J.K. , Rodionov V. , Han D.K.
Sci. Signal.2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Leukemic T-cell.
60.

"NEK11: linking CHK1 and CDC25A in DNA damage checkpoint signaling."
Soerensen C.S. , Melixetian M. , Klein D.K. , Helin K.
Cell Cycle9:450-455(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN CDC25A DEGRADATION
61.

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-286; SER-296 AND SER-301;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
62.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
63.

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T. , Prokhorova T.A. , Akimov V. , Henningsen J. , Johansen P.T. , Kratchmarova I. , Kassem M. , Mann M. , Olsen J.V. , Blagoev B.
Sci. Signal.4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
64.

"The 1.7 A crystal structure of human cell cycle checkpoint kinase Chk1: implications for Chk1 regulation."
Chen P. , Luo C. , Deng Y. , Ryan K. , Register J. , Margosiak S. , Tempczyk-Russell A. , Nguyen B. , Myers P. , Lundgren K. , Kan C.-C. , O'Connor P.M.
Cell100:681-692(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 1-289
65.

"Structural basis for Chk1 inhibition by UCN-01."
Zhao B. , Bower M.J. , McDevitt P.J. , Zhao H. , Davis S.T. , Johanson K.O. , Green S.M. , Concha N.O. , Zhou B.-B.S.
J. Biol. Chem.277:46609-46615(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 1-289
66.

"Structure-based design of novel Chk1 inhibitors: insights into hydrogen bonding and protein-ligand affinity."
Foloppe N. , Fisher L.M. , Howes R. , Kierstan P. , Potter A. , Robertson A.G.S. , Surgenor A.E.
J. Med. Chem.48:4332-4345(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-289
67.

"Patterns of somatic mutation in human cancer genomes."
Greenman C. , Stephens P. , Smith R. , Dalgliesh G.L. , Hunter C. , Bignell G. , Davies H. , Teague J. , Butler A. , Stevens C. , Edkins S. , O'Meara S. , Vastrik I. , Schmidt E.E. , Avis T. , Barthorpe S. , Bhamra G. , Buck G. , more...
Nature446:153-158(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS [LARGE SCALE ANALYSIS] VAL-223 AND MET-312

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